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Beckman Scholar Nicole M. Carter Publishes: Transcription factor NF-κB is modulated by symbiotic status in a sea anemone model of cnidarian bleaching

Beckman Scholar Nicole M. Carter Publishes: Transcription factor NF-κB is modulated by symbiotic status in a sea anemone model of cnidarian bleaching

  • Tuesday, December 12, 2017

Beckman Scholar Nicole M. Carter Publishes: Transcription factor NF-κB is modulated by symbiotic status in a sea anemone model of cnidarian bleaching

Learn more about what Beckman Scholar Nicole Carter has been working on at Boston University:

ABSTRACT

Transcription factor NF-κB plays a central role in immunity from fruit flies to humans, and NF-κB activity is altered in many human diseases. To investigate a role for NF-κB in immunity and disease on a broader evolutionary scale we have characterized NF-κB in a sea anemone (Exaiptasia pallida; called Aiptasia herein) model for cnidarian symbiosis and dysbiosis (i.e., “bleaching”). We show that the DNA-binding site specificity of Aiptasia NF-κB is similar to NF-κB proteins from a broad expanse of organisms. Analyses of NF-κB and IκB kinase proteins from Aiptasia suggest that non-canonical NF-κB processing is an evolutionary ancient pathway, which can be reconstituted in human cells. In Aiptasia, NF-κB protein levels, DNA-binding activity, and tissue expression increase when loss of the algal symbiont Symbiodinium is induced by heat or chemical treatment. Kinetic analysis of NF-κB levels following loss of symbiosis show that NF-κB levels increase only after Symbiodinium is cleared. Moreover, introduction of Symbiodinium into naïve Aiptasia larvae results in a decrease in NF-κB expression. Our results suggest that Symbiodinium suppresses NF-κB in order to enable establishment of symbiosis in Aiptasia. These results are the first to demonstrate a link between changes in the conserved immune regulatory protein NF-κB and cnidarian symbiotic status.

 

CONTINUE READING:

www.nature.com/scientificreports

DOI:10.1038/s41598-017-16168-w

Mansfield KM, NM Carter, L Nguyen, PA Cleves, A Alshanbayeva, LM Williams, A Penvose, JR Finnerty, VM Weis, T Siggers & TD Gilmore. 2017. Transcription factor NF-kappaB is modulated by symbiotic status in a sea anemone model of cnidarian bleaching. Scientific Reports 7: 16025


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