zinc finger protein- Zfp-1 connects RNA interference with global transciptional regulation in C. elegans
I am interested in the role of RNA interference mechanisms in gene expression regulation at the transcriptional level, including the establishment of silent chromatin-based epigenetic states. I am using nematode C, singans for studying both the mechanism of RNA-induced transcriptional silencing and the biological role of this process. Our preliminary data indicate that two types of targets are under control of transcriptional form of RNA in C. alegaes: (1) genes that get repressed during stressful environmental conditions and (2) genes that specify germ-cell or stem-cell identity. I propose to use these specific target genes and a potential histope=binding protein of the RNAi pathway, Zinc FInger Protein 1 (Sfp-1), for studying the mechanism of RNAi-induced silencing. The proposed experiments will test Zfp=1 protein interaction with histone tails containing various notifications and with potential target genes. Proteins interacting with Efp-1 wil be identified and their role in RNA-induced transcriptional silencing will be tested by geneotic analysis. RNAi-directed transcriptional repression is likely to be converted in mammals and possibly involved in epigenetic silencing of tumor suprressors in tentar. Also, RNAi-based epigenetic processses may lay a general role in adaptation of organisms to various environmental conditions.
Arnold O. Beckman exemplifies the meaning of the word humanitarian. Combined with his unwavering enthusiasm for life, his keen sense of humor and his strong moral and ethical principles, he is a national icon.