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Alla Grishok

Alla Grishok
Program
Beckman Young Investigators

Award Year
2008

Institution
Columbia University

Email:
ag2691@columbia.edu

Website:
http://cpmcnet.columbia.edu/dept/gsas/biochem/faculty/Grishok.html

Research Title:
zinc finger protein- Zfp-1 connects RNA interference with global transciptional regulation in C. elegans

Abstract:
I am interested in the role of RNA interference mechanisms in gene expression regulation at the transcriptional level, including the establishment of silent chromatin-based epigenetic states. I am using nematode C, singans for studying both the mechanism of RNA-induced transcriptional silencing and the biological role of this process. Our preliminary data indicate that two types of targets are under control of transcriptional form of RNA in C. alegaes: (1) genes that get repressed during stressful environmental conditions and (2) genes that specify germ-cell or stem-cell identity. I propose to use these specific target genes and a potential histope=binding protein of the RNAi pathway, Zinc FInger Protein 1 (Sfp-1), for studying the mechanism of RNAi-induced silencing. The proposed experiments will test Zfp=1 protein interaction with histone tails containing various notifications and with potential target genes. Proteins interacting with Efp-1 wil be identified and their role in RNA-induced transcriptional silencing will be tested by geneotic analysis. RNAi-directed transcriptional repression is likely to be converted in mammals and possibly involved in epigenetic silencing of tumor suprressors in tentar. Also, RNAi-based epigenetic processses may lay a general role in adaptation of organisms to various environmental conditions.

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