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Herschel Wade

Herschel Wade
Program
Beckman Young Investigators

Award Year
2009

Institution
Johns Hopkins University

Email:
herschel.wade@jhmi.edu

Website:
http://biophysics.med.jhmi.edu/BIOPHYS/wade.html

Research Title:
An Integrative Analysis of Multidrug Recognition and Multidrug signaling

Abstract:
The action of multidrug (MD) efflux pumps and their transcriptional regulators present new questions in the area of allostery due to the fact that their functional properties are coupled to not one, but numerous structurally diverse ligands. My research focuses on better understanding the mechanisms of MD recognition and MD-dependent control of protein conformation through studies on BmrR, a ligand promiscuous transcription factor with a key role in multidrug resistance (MDR) in B. subtilis. At our disposal, we have a battery of tools to facilitate a quantitative dissection of MD recognition and MD-directed allostery at a level not obtained for any MDR system to date. Our goal is to combine biochemical, thermodynamic and structural studies into an integrative analysis of MDR function. Discoveries from our studies will have general implications in understanding how to control multidrug-dependent signaling, a key component of bacterial and cellular multidrug resistance.

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