Multidimensional Ultrafast Optical-Ultrasonics Interferometric Microscopy: A New Light on Biological Imaging
Understanding the real time dynamics of living biological systems with high temporal and spatial resolutions as well as constituent specificity has become increasingly important for both research on regular in vivo biostructures and dynamical biochemical process at the molecular and cellular level, and on clinical diagnostics and pharmaceutical development. This proposal presents a development plan for a novel advanced microscopy combining the power of optical techniques (scanning optics and time resolved spectroscopy) with the biological responsiveness of ultrasonic waves, which potentially can eliminate the limitations associated with current pure optical and pure ultrasonic techniques. This new instrument will meet the key requirements imposed on ideal biological imaging techniques for the visualization of living biological specimens and processes including non-ionization and non-invasiveness, nanometer spatial resolution, high imaging sensitivity, deep (centimeter) penetration capability, free from labels, constituent specificity, truly 3D structural and compositional mapping, femto-/pico- second temporal resolution. This tool will open up new avenues of research in bioscience. We will address several fundamental issues to demonstrate the power of proposed technique including visualization of lipid and cell structures and early diagnosis of malignant tumors, and we intend to aggressively expand this technique for more complex and intriguing dynamic biophysical/biochemical processes as well as for practical biomedicine applications.
Arnold O. Beckman exemplifies the meaning of the word humanitarian. Combined with his unwavering enthusiasm for life, his keen sense of humor and his strong moral and ethical principles, he is a national icon.