Synthetic Biology Approach to Scaffolding Pathways for Small Molecule Biosynthesis
UNC, Chapel Hill
School of Pharmacy, Chemical Biology & Medicinal Chemistry
Thiazolyl peptides are ribosomally synthesized and post-translationally modified peptide (RiPP) natural products. Their biosynthesis involves the remarkable coordinated activity of a series of post-translational modifying enzymes, all directed by interaction with a leader peptide motif, which is part of the peptide precursor. Here we describe the complete in vitro reconstitution of one of these pathways and first steps in application towards the directed evolution of new variants of the natural product.