Assessing the Osteogenic Differentiation of Human Mesenchymal Stem Cells Co-Cultured with Human Umbilical Vein Endothelial Cells on a Peptide Amphiphile Nanomatrix
University of Alabama at Birmingham
The goal of this study was to evaluate the effects of crosstalk between human mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells (HUVECs) in a peptide amphiphile (PA) scaffold in vitro. The PA scaffolds has emerged as a viable option for bone tissue regeneration . We have developed the native bone extracellular matrix (ECM) mimicking microenvironment using PAs as an organic component, hMSCs as a osteogenic component,, and HUVECs as an angiogenic component.We have already demonstrated that PA-RGDS enhanced the osteogenic differentiation of hMSCs without aid of osteogenic supplement media and with synergistic effects by osteogenic supplement media, In addition, we hypothesized that cross-talk between co-cultured HUVECs and hMSCs would exhibit the best osteogenic and angiogenic responses, compared to HUVECs or hMSCs alone. As control groups, hMSCs were cultured separately in osteogenic supplement media, while HUVECs were cultured separately in endothelium basal media. Three different conditions were tested: 1) PA-RGDS nanomatrix, 2) PA-S nanomatrix, and 3) tissue culture plate. The cells were collected at 1, 7, 14, and 28 days and analyzed using Picogreen and alkaline phosphatase assays. Von Kossa staining was also performed after 28 days. PA-RGDS nanomatrix with co-cultured HUVECs or hMSCs condition shows increased angiogenesis, mineralization, and osteogenic differentiation.